Polymorphism and Protein Expression of p73 Gene in Relation to ...

posted by:

Cancer Research

[Abstract]

Objective : To study the distribution of p73 gene polymorphism in patients with colorectal tumor in Fujian Province, and explore the effect of p73 protein express on the development and progression in colorectal tumor.Method : All cases are from the patients who have received endoscopy and surgery in Fujian Provice Hospital.The samples from biopsy with endoscopy or surgery resection are confirmed colorectal adenocarcinoma or adenoma, the colorectal cancer is sporadic excluding Hereditary Non-Polyposis Colorectal Cancer(HNPCC). Recording patient?s age, sex, susceptive factors to colorectal cancer in details. Blood sample acquitted from patients were divided into three groups, colorectal adenocarcinoma group (n= 60), adenoma group (n=60) and healthy volunteer group (n=60) respectively. Tissue sample acquitted from patients were divided into three groups, colorectal adenocarcinoma group (n=30), adenoma group (n=30) and the distant normal mucosa group (n=30) respectively. PCR?restriction fragment length polymorphism (RFLP) method was used to analyze p73 gene polymorphism. Immunohistochemistry staining method was used to show the expression status of p73 protein. Finally, the above results were tested by x2 and Fisher exact probabilities statistical analysis.Result:1.The frequencies of the p73 genotypes were 43.3% for wild-type (GC/ GC), 31.7% for heterozygotes (GC/AT) and 25% for variants (AT/AT) in colorectal adenocarcinoma patients,55.0 ,26.7and 18.3% in colorectal adenoma patients, 60.0,35.0 and 5.0% in normal control ,respectively. There were significant differences among colorectal adenocarcinoma, colorectal adenoma and normal control.(p0.05)3.The expression of p73 protein.The case amount of p73 protein expression increase was 10 (43.3%) in colorectal adenocarcinoma tissues, 3 (10.0%) in colorectal adenoma tissues, 2 (6.7%) in normal tissues . The expression of p73 protein in colorectal adenocarcinoma tissues was significant higher than in colorectal adenoma tissues and in normal tissues .(p0.05).Conclusion:1. There were relationship between p73 gene polymorphism and colorectal tumor, the AT/AT homozygotes may have a greater risk of developing colorectal tumor in Fujian Province.2. Increase of p73 protein expression in relation to the development and progression of colorectal cancer.

Title: Polymorphism and Protein Expression of p73 Gene in Relation to Colorectal Tumor in Fujian Province

Category: Cancer Science

Filename: Polymorphism and Protein Expression of p73 Gene in Relation to Colorectal Tumor in Fujian Province.pdf

Pages: 160

Price: US$48.00

Buy This Paper:

Download:

Donated for this research subject:

FAVORITE: ADD TO FAVORITE

Contact: E-Mail:bizdg@126.com

????????????????TEL:1-888-774-999A

How to get this paper's electronic documents?

1, Click the "Buy Now" button to complete the online payment

2, Download the paper's electronic document from the successful payment return page/Or the system will send this paper's electronic document to your E-Mail within 24 hours

Version:zh-cn

Perhaps You will be interested in these papers

Source: http://www.rescancer.com/cancer-science/29460.html

home affordable refinance program harp harp world series game 5 moammar gadhafi harry connick jr rightnow